细粒棘球绦虫原头蚴microRNA表达谱分析

doi:10.11843/j.issn.0366-6964.2018.11.020

Abstract

Abstract:

The purpose of this study was to analyze the expression profile characteristics of miRNA of the protoscoleces in Echinococcus granulosus and to provide theoretical evidence for further revealing the role of miRNA in the development of the protoscoleces. The protoscoleces were collected from the infected sheep liver under sterile condition, and the total RNA was extracted. Then the miRNA Library of the protoscoleces was constructed and sequenced by RNA sequencing technology. The results showed that 109 known miRNA molecules were isolated from the protoscoleces, and 189 novel miRNA hairpin precursors were detected in the PSC of the E. granulosus. The target gene prediction results showed that these known miRNAs had 132 target genes, and the novel miRNAs had 3 152 target genes. A further study found that these miRNA molecules are widely involved in growth and development of key signaling pathways, such as Wnt, cAMP, Hedgehog, NF-κB, Notch and bile salt pathway. miRNA molecules were further researched by real-time quantitative PCR and in situ hybridization. The results suggested that there may be potential regulatory relationships between HG328413.1_36258 and β-catenin, HG328414.1_36364* and dis, as well as HG328399.1_25846 and axin. To sum up, a large number of new miRNA molecules were found in the E. granulosus in this study, which enriched the data of miRNA of the protoscoleces, and laid a foundation for further analysis of the role of miRNA in the development of protoscoleces in E. granulosus.

CLC Number:

S852.734

WANG Zheng-rong, BO Xin-wen, ZHANG Yan-yan, MA Xun, LU Ping-ping, XU Meng-fei, MENG Ji-meng. microRNA Profile Analyses of the Protoscoleces in Echinococcus granulosus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2477-2485.

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microRNA Profile Analyses of the Protoscoleces in Echinococcus granulosus

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